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Registros recuperados : 25 | |
2. | | MALABARBA, J.; BUFFON, V.; PASQUALI, G.; REVERS, L. F. Estudo estrutural e funcional do gene VvAGL11 e seu papel na morfogênese de sementes de Vitis vinifera. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 12., ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 8., 2014, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2014. p. 39 Biblioteca(s): Embrapa Uva e Vinho. |
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3. | | FRÂNCIO, L.; MALABARBA, J.; BUFFON, V.; REVERS, L. F. Desenvolvimento de marcadores para a seleção assistida de ausência de sementes em videiras no programa de melhoramento da Embrapa Uva e Vinho. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 14. ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 10., 2016, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa uva e Vinho, 2016. p. 56. (Embrapa Uva e Vinho. Documentos, 99) Biblioteca(s): Embrapa Uva e Vinho. |
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6. | | MALABARBA, J.; WAIRICH, A.; BUFFON, V.; CZERMAINSKI, A. B. C.; REVERS, L. F. Avaliação de marcadores SSR com potencial para seleção assistida de videiras apirências e resistentes ao míldio. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 10.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 6., 2012, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2012. p. 12. Resumo. Biblioteca(s): Embrapa Uva e Vinho. |
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7. | | MALABARBA, J.; WAIRICH, A.; BUFFON, V.; CZERMAINSKI, A. B. C.; REVERS, L. F. Avaliação de marcadores SSR com potencial para seleção assistida de videiras apirênicas e resistentes ao míldio. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, p. 4401-4404, 2012. Biblioteca(s): Embrapa Uva e Vinho. |
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9. | | WAIRICH, A.; MALABARBA, J.; BUFFON, V.; PORTO, D. D.; REVERS, L. F. Análise do perfil transcricional de genes candidatos associados à resistência ao míldio em videira, presentes no locus RPV3. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, p. 4332-4335, 2012. Biblioteca(s): Embrapa Uva e Vinho. |
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13. | | WAIRICH, A.; MALABARBA, J.; BUFFON, V.; PORTO, D. D.; REVERS, L. F. Perfil transcricional de genes candidatos associados à resistência ao P. viticola na cultivar resistente Villard Blanc e na cultivar suscetível Cabernet Sauvignon. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 10.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 6., 2012, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2012. p. 11. Resumo. Biblioteca(s): Embrapa Uva e Vinho. |
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14. | | MARIMON, P.; FRÂNCIO, L.; MALABARBA, J.; BUFFON, V.; REVERS, L. F. Utilização de um vetor viral para expressão ectópica do gene MrRGA8 em videira. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 15., 2017.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 11., 2017, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa Uva e Vinho, 2017. p. 39. Biblioteca(s): Embrapa Uva e Vinho. |
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15. | | MALABARBA, J.; BUFFON, V.; FRÂNCIO, L.; MARASCHIN, F. S.; PASQUALI, G.; MÁRGIS-PINHEIRO, M. N.; REVERS, L. F. Avaliação da função de VvAGL11 na morfogênese da semente da videira. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 14. ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 10., 2016, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa uva e Vinho, 2016. p. 46. (Embrapa Uva e Vinho. Documentos, 99) Biblioteca(s): Embrapa Uva e Vinho. |
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16. | | MALABARBA, J.; BUFFON, V.; WAIRICH, A.; GAETA, M. L.; MARIATH, J. E. A.; DORNELAS, M. C.; REVERS, L. F. Gene expression analysis of VVAG3 in grapevine reproductive tissues by in situ hybridization. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: Sociedade Brasileira de Genética, 2013. p. 3. Resumo. Biblioteca(s): Embrapa Uva e Vinho. |
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17. | | MALABARBA, J.; BUFFON, V.; MARIATH, J. E. A.; MARASHIN, F. S.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.). Plant Science, v. 269, p. 126-135, 2018. Biblioteca(s): Embrapa Uva e Vinho. |
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19. | | MALABARBA, J.; BUFFON, V.; MARIATH, J. E. A.; GAETA, M. L.; DORNELAS, M. C.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. The MADS-box gene Agamous-like 11 is essential for seed morphogenesis in grapevine. Journal of Experimental Botany, v. 68, n. 7, p. 1493-1506, mar. 2017. Biblioteca(s): Embrapa Uva e Vinho. |
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20. | | MALABARBA, J.; BUFFON, V.; FRÂNCIO, L.; MARIATH, J. E. A.; GAETA, M. L.; DORNELAS, M. C.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. The transcription of agamous-like 11 is essential for seed morphogenesis in grapevine. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstracts. [S.l.]: International Society for Plant Molecular Biology, 2015. p. 27. Biblioteca(s): Embrapa Uva e Vinho. |
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Registros recuperados : 25 | |
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Registro Completo
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
11/06/2019 |
Data da última atualização: |
06/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
FALAVIGNA, V. da S.; MALABARBA, J.; SILVEIRA, C. P.; BUFFON, V.; MARIATH, J. E. de A.; PASQUALI, G.; MARGIS-PINHEIRO, M.; REVERS, L. F. |
Afiliação: |
Vítor da Silveira Falavigna, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil; Jaiana Malabarba, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, BraziL; Carolina Pereira Silveira, Embrapa Uva e Vinho, Rua Livramento, 515, P.O. Box 130, Bento Gonçalves, RS 95701-008, Brazil; VANESSA BUFFON, CNPUV; Jorge Ernesto de Araújo Mariath, Laboratory of Plant Anatomy, Botany Department, UFRGS, Porto Alegre 91501-970, Brazil; Giancarlo Pasquali, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil; Márcia Margis?Pinheiro, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil; LUIS FERNANDO REVERS, CNPUV. |
Título: |
Characterization of the nucellus-specific dehydrin MdoDHN11 demonstrates its involvement in the tolerance to water deficit. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Plant Cell Reports [online], 2019. |
DOI: |
10.1007/s00299-019-02428-8 |
Idioma: |
Inglês |
Conteúdo: |
Abstract Key message MdoDHN11 acts in the nucellus layer to protect the embryo and the endosperm from limited water availability during apple seed development.AbstractDehydrins (DHNs) are protective proteins related to several plant developmental responses that involve dehy-dration such as seed desiccation and abiotic stresses. In apple (Malus × domestica Borkh.), the seed-specific MdoDHN11was suggested to play important roles against dehydration during seed development. However, this hypothesis has not yet been evaluated. Within this context, several experiments were performed to functionally characterize MdoDHN11. In situ hybridization analysis during apple seed development showed that MdoDHN11 expression is confined to a maternal tissue called nucellus, a central mass of parenchyma between the endosperm and the testa. The MdoDHN11 protein was local-ized in the cytosol and nucleus. Finally, transgenic Arabidopsis plants expressing MdoDHN11 were generated and exposed to a severe water-deficit stress, aiming to mimic a situation that can occurs during seed development. All transgenic lines showed increased tolerance to water deficit in relation to wild-type plants. Taken together, our results provide evidences that MdoDHN11 plays important roles during apple seed development by protecting the embryo and the endosperm from limited water availability, and the mechanism of action probably involves the interaction of MdoDHN11 with proteins and other components in the cell.Keywords Apple · Dehydrin · Seed development · Transgenic expression · Water-deficit stress MenosAbstract Key message MdoDHN11 acts in the nucellus layer to protect the embryo and the endosperm from limited water availability during apple seed development.AbstractDehydrins (DHNs) are protective proteins related to several plant developmental responses that involve dehy-dration such as seed desiccation and abiotic stresses. In apple (Malus × domestica Borkh.), the seed-specific MdoDHN11was suggested to play important roles against dehydration during seed development. However, this hypothesis has not yet been evaluated. Within this context, several experiments were performed to functionally characterize MdoDHN11. In situ hybridization analysis during apple seed development showed that MdoDHN11 expression is confined to a maternal tissue called nucellus, a central mass of parenchyma between the endosperm and the testa. The MdoDHN11 protein was local-ized in the cytosol and nucleus. Finally, transgenic Arabidopsis plants expressing MdoDHN11 were generated and exposed to a severe water-deficit stress, aiming to mimic a situation that can occurs during seed development. All transgenic lines showed increased tolerance to water deficit in relation to wild-type plants. Taken together, our results provide evidences that MdoDHN11 plays important roles during apple seed development by protecting the embryo and the endosperm from limited water availability, and the mechanism of action probably involves the interaction of MdoDHN11 with proteins and other components in the cell.Keywor... Mostrar Tudo |
Palavras-Chave: |
Transgenic expression; Water-deficit stress. |
Thesaurus NAL: |
Apples; Dehydrins; Seed development. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198444/1/Falavigna2019-Article-CharacterizationOfTheNucellus-.pdf
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Marc: |
LEADER 02425naa a2200277 a 4500 001 2109788 005 2020-01-06 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1007/s00299-019-02428-8$2DOI 100 1 $aFALAVIGNA, V. da S. 245 $aCharacterization of the nucellus-specific dehydrin MdoDHN11 demonstrates its involvement in the tolerance to water deficit.$h[electronic resource] 260 $c2019 520 $aAbstract Key message MdoDHN11 acts in the nucellus layer to protect the embryo and the endosperm from limited water availability during apple seed development.AbstractDehydrins (DHNs) are protective proteins related to several plant developmental responses that involve dehy-dration such as seed desiccation and abiotic stresses. In apple (Malus × domestica Borkh.), the seed-specific MdoDHN11was suggested to play important roles against dehydration during seed development. However, this hypothesis has not yet been evaluated. Within this context, several experiments were performed to functionally characterize MdoDHN11. In situ hybridization analysis during apple seed development showed that MdoDHN11 expression is confined to a maternal tissue called nucellus, a central mass of parenchyma between the endosperm and the testa. The MdoDHN11 protein was local-ized in the cytosol and nucleus. Finally, transgenic Arabidopsis plants expressing MdoDHN11 were generated and exposed to a severe water-deficit stress, aiming to mimic a situation that can occurs during seed development. All transgenic lines showed increased tolerance to water deficit in relation to wild-type plants. Taken together, our results provide evidences that MdoDHN11 plays important roles during apple seed development by protecting the embryo and the endosperm from limited water availability, and the mechanism of action probably involves the interaction of MdoDHN11 with proteins and other components in the cell.Keywords Apple · Dehydrin · Seed development · Transgenic expression · Water-deficit stress 650 $aApples 650 $aDehydrins 650 $aSeed development 653 $aTransgenic expression 653 $aWater-deficit stress 700 1 $aMALABARBA, J. 700 1 $aSILVEIRA, C. P. 700 1 $aBUFFON, V. 700 1 $aMARIATH, J. E. de A. 700 1 $aPASQUALI, G. 700 1 $aMARGIS-PINHEIRO, M. 700 1 $aREVERS, L. F. 773 $tPlant Cell Reports [online], 2019.
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